DOCUMENTED BY BATCHBPC-157 is a synthetic pentadecapeptide catalogued for laboratory research reference. This profile summarises its chemical identity, primary structure, molecular constants, physical characteristics, and the analytical methods used to confirm identity and purity per batch. It contains no use, handling, or outcome information and is intended solely as a bench reference document.
Identity and nomenclature
BPC-157 is registered under CAS number 137525-51-0 and corresponds to PubChem CID 9941957. The designation “BPC” originates from the laboratory nomenclature “Body Protection Compound,” the name assigned by the researchers who first characterised the sequence; the compound is also referenced in the literature as bepecin and PL 14736. The sequence corresponds to a partial fragment of a protein originally isolated from gastric juice, and modern research material is produced by solid-phase peptide synthesis rather than extraction. When ordering research material, the CAS number and the full amino-acid sequence are the two identifiers that unambiguously specify the compound, since abbreviations and trade names vary between suppliers.
Primary structure and sequence
BPC-157 is a linear peptide of fifteen amino-acid residues (a pentadecapeptide). Its primary sequence, from the N-terminus to the C-terminus, is:
- Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val
- In single-letter notation: GEPPPGKPADDAGLV
The chain contains three consecutive proline residues near the N-terminus and no cysteine, so the molecule carries no intramolecular disulfide bridge and exists as an unmodified linear sequence. Confirming the residue order against this reference sequence is the first step in verifying that received material is the intended compound rather than a truncation or substitution variant.
Molecular formula and weight
The molecular formula of BPC-157 is C62H98N16O22, giving a monoisotopic and average molecular weight of approximately 1419.53 g/mol (reported in the literature as 1419.53 to 1419.556 depending on the isotope convention used). This mass is the primary target value in mass-spectrometric identity confirmation: an observed mass that deviates from the expected 1419.53 by more than the instrument’s stated tolerance indicates an impurity, an adduct, or an incorrect sequence. Because the peptide is unmodified and un-conjugated, the theoretical mass can be calculated directly from the sequence and compared against the measured value with no correction for salts or protecting groups.
Physical appearance and bench solubility
Research-grade BPC-157 is typically supplied as a lyophilised (freeze-dried) white to off-white powder, sealed in a vial under an inert or reduced-pressure headspace. Lyophilised peptide is generally stored frozen and protected from light and moisture; solution preparation for analytical or bench work is performed with an appropriate aqueous solvent immediately before use, with dissolved material kept refrigerated for short-term handling and frozen for longer intervals. The presence of two acidic aspartate and one glutamate residue makes the peptide readily soluble in water and mildly basic buffers, which is relevant when preparing samples for chromatography or spectrometry. These handling notes describe bench storage conditions only and carry no implication of use beyond the laboratory.
Analytical verification: LC-MS, HRMS and HPLC
Two orthogonal methods establish that a batch matches its stated identity and purity. Liquid chromatography coupled to mass spectrometry (LC-MS), and high-resolution mass spectrometry (HRMS), confirm identity by measuring the intact molecular mass against the theoretical 1419.53 g/mol and, where fragmentation is used, by matching sequence-specific fragment ions. Reverse-phase high-performance liquid chromatography (HPLC) confirms purity by resolving the main peptide peak from synthesis-related impurities and reporting the main peak as a percentage of total peak area. At livvmore, research peptides are identity- and purity-tested at the University of Southern California’s Alfred E. Mann Multi-Omics Mass Spectrometry Core by LC-MS/HRMS on a per-batch basis, and the corresponding certificate is published for each lot. The same testing framework applies across the catalogue, including peptides such as GHK-Cu and MOTS-C. Details of the methodology are documented on the quality and testing page.
Reading a Certificate of Analysis
A Certificate of Analysis (COA) for a peptide such as BPC-157 should let a researcher reconstruct the identity independently. Key fields to read are: the stated sequence (compared against GEPPPGKPADDAGLV), the theoretical versus observed mass (compared against 1419.53 g/mol), the analytical method named (for example LC-MS or HRMS for identity and HPLC for purity), the reported purity percentage with its integration basis, the batch or lot number, and the issuing laboratory. A COA that names an independent testing facility, states the method, and reports both an observed mass and a purity figure is more informative than one that lists a nominal purity without supporting data. Cross-checking the observed mass and sequence on the COA against the reference constants above is the fastest way to confirm that received material is the intended compound.
For laboratory research use only. Not for human or veterinary use.
Frequently asked questions
What is the molecular weight and formula of BPC-157?
BPC-157 has a molecular formula of C62H98N16O22 and an average molecular weight of approximately 1419.53 g/mol. It is registered under CAS number 137525-51-0 (PubChem CID 9941957) and is a linear peptide of 15 amino-acid residues.
What is the amino acid sequence of BPC-157?
BPC-157 is a pentadecapeptide with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val, written in single-letter notation as GEPPPGKPADDAGLV. It is a linear chain with three consecutive proline residues near the N-terminus and no cysteine, so it contains no disulfide bond.
How is BPC-157 identity confirmed in a laboratory?
Identity is confirmed by mass spectrometry, typically LC-MS or high-resolution mass spectrometry (HRMS), which measures the intact molecular mass against the theoretical value of about 1419.53 g/mol for BPC-157. Purity is measured separately by reverse-phase HPLC. At livvmore, BPC-157 batches are tested at the USC Alfred E. Mann Multi-Omics Mass Spectrometry Core, with a certificate published per lot.
What does the CAS number 137525-51-0 refer to?
CAS number 137525-51-0 is the Chemical Abstracts Service registry identifier for BPC-157, the synthetic pentadecapeptide with sequence GEPPPGKPADDAGLV and molecular formula C62H98N16O22. Quoting this CAS number alongside the sequence unambiguously specifies the compound regardless of the trade name or abbreviation used.
Is BPC-157 the same kind of peptide as retatrutide or semaglutide?
No. BPC-157 (CAS 137525-51-0, 15 residues, molecular formula C62H98N16O22, MW ~1419.53) is a structurally distinct sequence and is chemically unrelated to that peptide family. Retatrutide (GLP3-RT, CAS 2381089-83-2), tirzepatide (GLP2-TZ) and semaglutide (GLP1-SM) are separate, larger peptide sequences catalogued under the GLP collection; BPC-157 is a distinct compound with its own identity and per-batch testing profile.